NAD+ 500mg

Research applications

• Mitochondrial function and cellular respiration studies
• Sirtuin and PARP enzyme activity research
• Cellular ageing and senescence models
• DNA repair signalling pathway research

What the literature reports on NAD+

NAD+ functions as a coenzyme in hundreds of redox reactions and as a required substrate for NAD-consuming enzymes, including the sirtuin family (SIRT1-7) and poly(ADP-ribose) polymerases (PARPs). Because both enzyme families are implicated in metabolic regulation and genomic stability, NAD+ availability is a recurring variable in the ageing and metabolic-disease research literature.

Cellular ageing research

Multiple animal studies have reported an age-related decline in NAD+ levels across tissues including liver, muscle and brain, alongside a corresponding decline in markers of mitochondrial biogenesis and function. Research groups have used direct NAD+ administration and precursor-based approaches (nicotinamide mononucleotide, nicotinamide riboside) as tools to study whether restoring NAD+ pools can reverse or slow these age-associated changes in animal models.

Mitochondrial function studies

Because NAD+ is a direct substrate in the electron transport chain, in vitro and animal studies have examined its role in maintaining mitochondrial membrane potential and oxidative phosphorylation capacity. This research has been extended to disease models involving mitochondrial dysfunction, including studies of metabolic syndrome and neurodegeneration in rodent models.

DNA repair and genomic stability

PARP enzymes consume NAD+ as a substrate during DNA repair processes, and studies have reported that NAD+ depletion under conditions of high DNA damage can impair repair capacity in cultured cells. This line of research connects NAD+ metabolism research to broader studies of genomic stability and cellular stress response.

Precursor pathway research

Because direct NAD+ administration faces cell-permeability constraints in some experimental systems, a parallel research literature examines NAD+ precursor molecules, including nicotinamide mononucleotide (NMN) and nicotinamide riboside (NR), as alternative tools for raising intracellular NAD+ pools. Comparative studies between direct NAD+ administration and precursor-based approaches are used to characterise differences in cellular uptake kinetics and downstream metabolic effects.

Metabolic syndrome research

Rodent models of diet-induced metabolic dysfunction have been used to study how NAD+ availability relates to markers of insulin sensitivity and hepatic lipid metabolism, with research reporting associations between NAD+ decline and metabolic syndrome phenotypes. This research thread situates NAD+ within the broader metabolic and ageing research literature relevant to this catalogue's LEAN and FOCUS research categories.

Laboratory handling

NAD+ is sensitive to hydrolysis and should be stored as lyophilised powder at -20°C, protected from light and moisture, until reconstitution. Because NAD+ in solution degrades more readily than many peptides, researchers typically prepare working solutions in small aliquots close to the time of use rather than storing large reconstituted volumes.

• Lyophilised powder: store at -20°C, protected from light and moisture.
• Reconstitution: use sterile water or an appropriate buffer per the researcher's protocol; prepare small aliquots to limit freeze-thaw exposure.
• Reconstituted solution: use promptly or store at -20°C in single-use aliquots.
• Each batch ships with a certificate of analysis confirming ≥99% purity by HPLC and identity by mass spectrometry.

See the peptide reconstitution and storage guide for general laboratory handling practice. This product is intended for laboratory research use only.