5-Amino-1MQ 50mg

Research applications

• NNMT enzyme inhibition and adipocyte metabolism research
• Cell-culture studies of methylation capacity and NAD+ salvage
• Rodent models of diet-induced adiposity
• Small-molecule structure-activity research in the quinolinium class

What the literature reports on 5-Amino-1MQ

NNMT catalyses the transfer of a methyl group from S-adenosylmethionine to nicotinamide, a reaction that has been reported to influence both cellular methylation capacity and the availability of nicotinamide for NAD+ salvage synthesis. 5-Amino-1MQ was identified in medicinal chemistry research as a selective, cell-permeable inhibitor of this enzyme.

Adipocyte metabolism research

Because NNMT is highly expressed in white adipose tissue, cell-culture studies using 5-Amino-1MQ have reported changes in adipocyte differentiation markers and lipid accumulation following NNMT inhibition. This research has extended to rodent models of diet-induced adiposity, where studies have reported changes in body composition parameters associated with NNMT inhibition in adipose tissue.

Methylation and NAD+ pathway research

Because NNMT consumes both SAM and nicotinamide, research has examined how its inhibition by 5-Amino-1MQ affects broader cellular methylation reactions and NAD+ salvage pathway flux. This connects 5-Amino-1MQ research to the wider literature on NAD+ metabolism and its role in cellular energy regulation.

Tool-compound research applications

As a selective small-molecule inhibitor, 5-Amino-1MQ is used across multiple laboratories as a pharmacological tool to dissect NNMT-dependent pathways from other metabolic regulators, a role documented in the broader medicinal chemistry literature on quinolinium-based enzyme inhibitors.

Comparative research with peptide-based metabolic compounds

Because 5-Amino-1MQ acts through a distinct, non-peptide mechanism relative to GIP/GLP-1 receptor agonists such as tirzepatide or mitochondrial signalling peptides such as MOTS-c, researchers studying adipose tissue metabolism have used it as a mechanistically independent tool compound, allowing NNMT-pathway effects to be examined separately from incretin-receptor or mitochondrial-signalling pathways within the same experimental systems.

Analytical identity research

Because the compound is registered under several related CAS numbers depending on salt form, analytical chemistry literature has examined the identity and purity characterisation of 5-Amino-1MQ preparations, an area of active documentation given the variation in salt-form labelling observed across commercial suppliers.

Laboratory handling

5-Amino-1MQ is supplied as a powder and should be stored at room temperature or -20°C per the batch certificate of analysis, protected from light and moisture. As a small molecule rather than a peptide, it is generally more stable at room temperature than the peptides in this catalogue, but researchers should follow the storage conditions specified on the batch COA for the specific salt form supplied.

• Powder: store as specified on the batch certificate of analysis, protected from light and moisture.
• Solubility: prepare stock solutions per the researcher's protocol using an appropriate solvent for the salt form supplied.
• Working solutions: prepare fresh where possible; document solvent and concentration for reproducibility.
• Each batch ships with a certificate of analysis confirming ≥99% purity by HPLC and identity by mass spectrometry.

This product is intended for laboratory research use only.