IGF-1 LR3 1mg

Research applications

• IGF-1 receptor / PI3K / Akt signalling pathway research
• In vitro cell proliferation and differentiation studies
• IGF-binding protein interaction research
• Comparative growth-factor signalling studies in animal models

What the literature reports on IGF-1 LR3

Native IGF-1 activity in vivo and in cell culture is substantially modulated by a family of IGF-binding proteins (IGFBPs), which sequester the growth factor and limit receptor engagement. IGF-1 LR3 was engineered specifically to reduce IGFBP affinity while preserving IGF-1 receptor binding, a design documented in the original biochemistry literature describing Long R3 IGF-1 analogues.

Cell signalling research

In vitro studies using IGF-1 LR3 have characterised downstream activation of the PI3K/Akt and MAPK/ERK signalling cascades following IGF-1 receptor engagement, using the reduced IGFBP interference of this analogue to obtain more consistent dose-response data than is typically achievable with native IGF-1 in serum-containing culture media.

Cell proliferation studies

Because IGF-1 receptor signalling is a well-characterised driver of cell proliferation and survival in multiple tissue types, IGF-1 LR3 has been used across cell-culture research to study proliferation, differentiation and apoptosis resistance in a range of cell lines, particularly in musculoskeletal and hepatic research contexts.

Animal model research

Animal studies using IGF-1 LR3 have examined growth-factor signalling in the context of skeletal muscle and connective tissue biology, with the analogue's extended bioavailability used as a tool to sustain receptor engagement over longer experimental windows than native IGF-1 permits.

Comparative growth-hormone-axis research

Because IGF-1 is a principal downstream mediator of growth hormone axis signalling, researchers frequently study IGF-1 LR3 alongside GHRH-analogue peptides such as CJC-1295 and tesamorelin, using IGF-1 LR3 as a downstream pathway tool to study receptor-level effects independent of upstream growth hormone secretagogue activity.

Identity and nomenclature research

Because multiple CAS numbers circulate in commercial literature for IGF-1 LR3, researchers sourcing this analogue for identity-sensitive work should cross-reference the batch certificate of analysis against primary manufacturer records, an identity-verification practice documented as necessary given inconsistencies observed across vendor product listings for this analogue.

Laboratory handling

IGF-1 LR3 is supplied as a lyophilised powder and should be stored at -20°C, protected from light and moisture, until reconstitution. As a larger recombinant protein, it is generally reconstituted with acidified sterile water or a manufacturer-specified buffer to improve solubility, following the protocol on the batch certificate of analysis.

• Lyophilised powder: store at -20°C, protected from light and moisture.
• Reconstitution: use the diluent specified on the batch COA; introduce slowly along the vial wall.
• Post-reconstitution: aliquot and store at -20°C; avoid repeated freeze-thaw cycles, which are more disruptive to larger proteins.
• Each batch ships with a certificate of analysis confirming ≥99% purity by HPLC and identity by mass spectrometry.

See the peptide reconstitution and storage guide for a full protocol reference. This product is intended for laboratory research use only.